Purifi cation and characterization of two phospho-β-galactosidases, LacG1 and LacG2, from Lactobacillus gasseri ATCC33323
نویسندگان
چکیده
Probiotics improve the intestinal microbiota and exert benefi ts for the host (Fuller, 1989; Parvez et al., 2006). Some strains of lactobacilli utilize lactose and alleviate lactose intolerance (Montalto et al., 2006). Lactobacillus gasseri is one of the predominant lactobacilli in the human intestine, and strains are used in probiotic foods. Lactobacillus gasseri is the major homofermentative species belonging in the B1 subgroup of the Lactobacillus acidophilus group found in the human gastrointestinal tract (Benno et al., 1989). Lb. gasseri survives in the stomach and small intestine (Pedrosa et al., J. Gen. Appl. Microbiol., 58, 11‒17 (2012)
منابع مشابه
Identification of lactose phosphotransferase systems in Lactobacillus gasseri ATCC 33323 required for lactose utilization.
Improving the annotation of sugar catabolism-related genes requires functional characterization. Our objective was to identify the genes necessary for lactose utilization by Lactobacillus gasseri ATCC 33323 (NCK334). The mechanism of lactose transport in many lactobacilli is a lactose/galactose-specific permease, yet no orthologue was found in NCK334. Characterization of an EI knockout strain [...
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